Afternoon flowers,

Having recently moved to Diva 6.1.1, we're experiencing some software
lock-up problems, usually after closing down CS&T, that require a
full re-install, which is becoming tiresome, and I wondered if any
others had experienced similar problems?

Wayne

Wayne Turnbull
Flow Cytometry Services
Department of Immunobiology
GKT Guy's Hospital
London SE1 9RT
Received on Wed Oct 15 12:38:00 2008
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Re: Diva 6.1.1 user issues
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From: Berend <b.hooibr...@amc.uva.nl>
Date: Thu Oct 16 2008 - 03:58:54 EDT

We have seen similar problems with 6.1.1 and CST module. I am not
able to run the cst when I started the diva software in a Windows
(normal)User account. It locks-up immediately. So, we first log in
as
administrator in windows xp, do the cst run, quit diva after this qc-
check and log in as (limited) user. Thereafter we startup diva again
and everything works fine, except the cst and configuration modules.
Our service engineer (BD) couldn't fix it so far. Still I prefer the
let everybody work as limited user with the LSR, to keep systems
clean from al kind of ?%^&b 9b  b " people want to install.
Berend

On Oct 15, 2008, at 3:43 PM, Nilles, Tricia L.. wrote:

> Wayne,

> Yes, we also have experienced a similar issue.     After running CS&T,
> we cannot make any
> changes (ie renaming specimens, experiments) we first have to exit
> and relaunch the
> software.  Logging out does not work.  I have discussed this with
> our local service rep
> and also one of the software specialists who came in for local
> training and demos, and no
> one has offered a 'fix'.

> Tricia L Nilles
> Research Specialist
> Flow Cytometry Laboratory
> Johns Hopkins Bloomberg School of Public Health
> Phone:     410-502-9290
> Fax:       410-614-2503

> -----Original Message-----
> From: Wayne Turnbull [mailto:wayne.turnb...@kcl.ac.uk]
> Sent: Tuesday, October 14, 2008 9:32 AM
> To: cyto-inbox
> Subject: Diva 6.1.1 user issues

> Afternoon flowers,

> Having recently moved to Diva 6.1.1, we're experiencing some software
> lock-up problems, usually after closing down CS&T, that require a
> full re-install, which is becoming tiresome, and I wondered if any
> others had experienced similar problems?

> Wayne

> Wayne Turnbull
> Flow Cytometry Services
> Department of Immunobiology
> GKT Guy's Hospital
> London SE1 9RT

Berend Hooibrink
Flow cytometry core facility
Dept.. Cellbiology & Histology room L3-115
AMC
Meibergdreef 15
1105AZ  Amsterdam
Netherlands
Tel.: 31(0)20-5666804
Fax: 31(0)20-6974156
Received on Thu Oct 16 15:38:00 2008
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RE: Diva 6.1.1 user issues
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From: Ted Hibbs <Ted_Hi...@bonfils.org>
Date: Thu Oct 16 2008 - 22:27:10 EDT

Berend,

We had a similar issue when we first got our BD CantoII with Diva
6.1.1.  It
is related to the permissions on the folders on the C:\ drive, though
I donb t
remember exactly where.  Once I changed the permissions on the
folders, it is
working well.

Ted

From: Berend [mailto:b.hooibr...@amc.uva.nl]
Sent: Thursday, October 16, 2008 1:59 AM
To: Cytometry Mailing List
Subject: Re: Diva 6.1.1 user issues

We have seen similar problems with 6.1.1 and CST module. I am not
able
to run
the cst when I started the diva software in a Windows (normal)User
account.
It locks-up immediately. So, we first log in as administrator in
windows xp,
do the cst run, quit diva after this qc-check and log in as (limited)
user.
Thereafter we startup diva again and everything works fine, except
the
cst
and configuration modules. Our service engineer (BD) couldn't fix it
so far.
Still I prefer the let everybody work as limited user with the LSR,
to
keep
systems clean from al kind of ?%^&b 9b  b " people want to install.

Berend

On Oct 15, 2008, at 3:43 PM, Nilles, Tricia L.. wrote:

Wayne,

Yes, we also have experienced a similar issue. After running CS&T, we
cannot
make any

changes (ie renaming specimens, experiments) we first have to exit
and
relaunch the

software.  Logging out does not work.  I have discussed this with our
local
service rep

and also one of the software specialists who came in for local
training and
demos, and no

one has offered a 'fix'.

Tricia L Nilles

Research Specialist

Flow Cytometry Laboratory

Johns Hopkins Bloomberg School of Public Health

Phone: 410-502-9290

Fax:  410-614-2503

-----Original Message-----

From: Wayne Turnbull [mailto:wayne.turnb...@kcl.ac.uk]

Sent: Tuesday, October 14, 2008 9:32 AM

To: cyto-inbox

Subject: Diva 6.1.1 user issues

Afternoon flowers,

Having recently moved to Diva 6.1.1, we're experiencing some software

lock-up problems, usually after closing down CS&T, that require a

full re-install, which is becoming tiresome, and I wondered if any

others had experienced similar problems?

Wayne

Wayne Turnbull

Flow Cytometry Services

Department of Immunobiology

GKT Guy's Hospital

London SE1 9RT

Berend Hooibrink

Flow cytometry core facility

Dept.. Cellbiology & Histology room L3-115

AMC

Meibergdreef 15

1105AZ  Amsterdam

Netherlands

Tel.: 31(0)20-5666804

Fax: 31(0)20-6974156

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RE: Diva 6.1.1 user issues
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From: Linkes, Sean Patrick <sean.lin...@utoledo.edu>
Date: Tue Oct 14 2008 - 16:11:56 EDT

No problems here. Our CS&T works wonderfully. Sometimes the setup
doesn't work right even with a freshly prepared tube of beads (with
the BD Field Service guy leaving one day prior having aligned the
lasers).        Sean

Sean Linkes MS
Research Associate
Flow Cytometry Core Manager
The University of Toledo HSC
(419) 383 3402
<http://hsc.utoledo.edu/depts/micro/UT_Flow_Cytometry.html>

________________________________

From: Wayne Turnbull [mailto:wayne.turnb...@kcl.ac.uk]
Sent: Tue 10/14/2008 9:32 AM
To: cyto-inbox
Subject: Diva 6.1.1 user issues

Afternoon flowers,

Having recently moved to Diva 6.1.1, we're experiencing some software
lock-up problems, usually after closing down CS&T, that require a
full re-install, which is becoming tiresome, and I wondered if any
others had experienced similar problems?

Wayne

Wayne Turnbull
Flow Cytometry Services
Department of Immunobiology
GKT Guy's Hospital
London SE1 9RT
Received on Wed Oct 15 14:18:00 2008
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RE: Diva 6.1.1 user issues
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From: Roberts, Daniel <Daniel.Robe...@covance.com>
Date: Wed Oct 15 2008 - 18:53:42 EDT

 I've been using 6.1.1 since May - no problems here. I'd question
your
Ram,
but if you bought the computer as part of the package - you should be
OK. I
know that sometimes leaving the beads on there can cause issues - but
If
memory serves (as opposed to habit) the CST software prompts/makes
you
take
the beads off. Other than that, I can't think of why it wouldn't
reconnect
to Diva.

Dan
Technical Resource Specialist
Covance Labs, NA
Vienna, VA  20850

-----Original Message-----
From: Linkes, Sean Patrick
To: cyto-inbox
Sent: 10/14/2008 3:11 PM
Subject: RE: Diva 6.1.1 user issues

No problems here. Our CS&T works wonderfully. Sometimes the setup
doesn't work right even with a freshly prepared tube of beads (with
the
BD Field Service guy leaving one day prior having aligned the
lasers).
Sean
Sean Linkes MS
Research Associate
Flow Cytometry Core Manager
The University of Toledo HSC
(419) 383 3402
 <http://hsc.utoledo.edu/depts/micro/UT_Flow_Cytometry.html>

  _____

From: Wayne Turnbull [mailto:wayne.turnb...@kcl.ac.uk]
Sent: Tue 10/14/2008 9:32 AM
To: cyto-inbox
Subject: Diva 6.1.1 user issues

Afternoon flowers,

Having recently moved to Diva 6.1.1, we're experiencing some software
lock-up problems, usually after closing down CS&T, that require a
full re-install, which is becoming tiresome, and I wondered if any
others had experienced similar problems?

Wayne

Wayne Turnbull
Flow Cytometry Services
Department of Immunobiology
GKT Guy's Hospital
London SE1 9RT

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Re: Fw: NEW FLOWCYTOMETRY SOFTWARE FOR ALL BD and Beckman/Coulter
Flow
Cytometers CAN PROCESS 24,000 SAMPLES PER HOUR...Standard Header|Full
Message View J. Paul Robinson J. Paul Robinson ...
 ViewFriday,September 28, 2007 9:37:32 AM
 To:mitchell haynes
Cc:da..._at_kanecki2.com; skel..._at_flowcyt.cyto.purdue.edu

Steve
what is this email - it came to me with Bob Murphy's name associated
with it.

 It seems to be an advertisement, this junk mail, and it seems
to have been modified by you ...

So I guess I am confused.

 was this sent to the list,

 or do you have an details about it

 - i am concerned about these junk messages going out to our members,
-

if they are using our lists, I will deal with them appropriately,

but I am not happy about this - any info you can give me appreciated

thanks

paul

TO ROBERT MURPHY  WITH LINKS FOR DOWNLOAD AND ALL INFORMATION
PERTAINING TO FCS CYTORPO QUICK FACS 11

SOME HOW IT WAS FOWARDED TO: J PAUL ROBINSON...MAYBE IT WAS
INTERCEPTED!

Fw: NEW FLOWCYTOMETRY SOFTWARE FOR ALL BD and Beckman/Coulter
Flow Cytometers CAN PROCESS 24,000- 48,000 SAMPLES PER HOUR
Standard
Header|Full Message
View
mitchell haynes <buybro..._at_yahoo.com>
ViewFriday,
September 28, 2007 1:36:36 AM
To:j..._at_flowcyt.cyto.purdue.edu
Cc:da..._at_kanecki2.com
Cytopro
quick facs savings analysis.doc (82KB); Setup-Quick
Facs 11 Demo.exe (7854KB); FCS
CYTOPRO BENIFITS.doc (25KB)
Find:
Dear Robert Murphy
NEW
FLOWCYTOMETRY SOFTWARE FOR ALL BD and Beckman/Coulter Flow Cytometers
CAN
PROCESS 24,000-42,000 SAMPLES PER HOUR.
FLOW CYTOMETRY    FCS CYTO PRO QUICK FACS
http://www.kanecki.com/flow.html
Increased quality and productivity. With 10,000,000 event files, you
can process 24,000 samples/hour, and maintain quality up to Sigma 5
or
better. Compare this to having your research technologist performing
only 100 samples/hour analysis.
Increased laboratory utilization by 3X because you can perform the
analysis off-lab and free laboratory time for reading samples. This
was achieved when I developed the program, and we had a program
project grant from 1992 to 1998 of $8M.
Works with FCS 3.0 in all data modes as floating point, integer*4,
and
ASCII.
Works with BD and Beckman/Coulter Flow Cytometers and Cell Sorters
Backwards compatible with FCS 2.0 files and Flow Cytometers and Cell
Sorters.
Can read FCS 3.0 files up to 10M events with 20 parameters.
Easy to Use, three step process. Load initial file, set gate, specify
file list to process. That's it.
Collaboration tools to allow you to cut and paste image results to
results.
Statistical analysis results imprinted on histogram plots directly as
mean, mode, and median with the ability to present results in log
mode
or linear mode, depending on the detector used.
Plain vanilla coding for easy update and maintenance to allow for the
greatest user and software quality.
One time purchase fee, no yearly renewal fees as with others.
Proven tract record in FACS, Fluorescent Activated Cell Sorter
Laboratory. The laboratory was rated the best laboratory in the
Midwest USA in 1990.
This application is designed for large-scale fluorescent activated
cell sorter
analysis. The program can read up to FCS 3.0 files and has been
tested
to run on Becton Dickinson and CoulterOrtho based flow cytometers and
cell sorters. The main advantage of this program is that you can have
the computer perform the analysis for you after you have selected the
region to analyze. The result is that up to 24,000 samples per hour
can be analyzed on a 1.4 GHz speed computer.
This program is designed for researcher and technologist use. It uses
rectangular gating, and is intuitive to use. To use this program, the
FCS must have the extension, *.bin as "54203023.bin" as an example.
The *.bin extension is what the computer uses to locate the files on
the computer.
THANK YOU FOR YOU TIME
MITCHELL HAYNES
VP
SALES
KANECKI
ASSOCIATES
832-347-1669

<buybroker_at_yahoo.com>

> To: skelley_at_flowcyt.cyto.purdue.edu
> Cc: skelley_at_flowcyt.cyto.purdue.edu
> Sent: Friday, September 28, 2007 11:39:02 AM
> Subject: NOT A JUNCK MAIL STEVE WAS JUST FOWARDING INFORMATION TO PROPER
> CHANNELS

> Dear Paul,

> I recieved your responce to the email I sent. Please understand it is not
> junkmail but a update on new technology that will inhance all
> flowcytometers

..It is currently being evaluated by BD who request for this
> software to be developed directly by our corporation.

> It was simply sent as an announcement for you concideration.
> The software is demonstrates precision and a higer processing rate than
> every existing software today.
> If you have any questions please call I provided my phone number with the
> email.

I understand institutions of your caliber is always looking for new
> technology.

Futhermoore this is the only software in the world that works
> for every platform on one peice of software
> Thank your for you time and have a great day.

> Please do not blame Steve for send the information to the proper channels

> I would think you would be upset if he did not foward important infomation
> that pertains to furthering cytometry breakthroughs.

> If you would like us to send information to another address that won't
> interfer please foward it to me and I will make sure that there are no
> more misunderstandings.

Mitchell Haynes

From: "jpr_at_flowcyt.cyto.purdue.edu"
<jpr_at_flowcyt.cyto.purdue.edu>

To: mitchell haynes <buybroker_at_yahoo.com>

Cc: jpr_at_flowcyt.cyto.purdue.edu

Sent: Friday, September 28, 2007 2:26:51 PM

Subject: Re: Fw: NOT A JUNCK MAIL STEVE WAS JUST FOWARDING
INFORMATION
TO
PROPER CHANNELS

Sorry,

I think it is junk mail

regards

paul robinson

> > On Nov 30, 2:01 pm, Larry Farrell <farrl...@isu.edu> wrote:
> >> Mitch Haynes wrote:
> >>> Why do you continue to post cytometry materials in misc.health.aids?
> >>> That is an HIV/AIDS newsgroup and your material is completely off
> >>> topic.  No one in the group is interested.
> >>> --
> >> [Multiple copies snipped]
> >> Very interesting.  I got a "Mail Undeliverable" notice when I sent
> >> this directly to Mr. Haynes but he turns around and posts multiple
> >> copies of a personal e-mail message into a newsgroup.  Extremely poor
> >> Netiquette (although it is just a further example of the poor
> >> Netiquette displayed by the issue about which I tried to contact him).
> >>     Exactly what is it that you are trying to accomplish, Mr. Haynes?
> >> --
> >> Larry D. Farrell, Ph.D.
> >> Professor of Microbiology
> >> Idaho State University
> >> --
> >> Posted via a free Usenet account fromhttp://www.teranews.com
> > PLEASE PROVIDE PROOF OF UNDELIVERALBE MAIL NOTICE...I GOT YOUR MAIL SO
> > DID PAUL ROBINSON....WHY DON'T YOU CARE....?
> > THAT IS THE PROBLEM....ONLY GOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOGLE WILL
> > PROVIDE THE INFORMATION TO THE WORLD...
> > ISSUE....WHY DID YOU NOTIFY J PAUL ROBINSON ABOUT POST?
> > IT IS GOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOLES WEBSITE........
> > I BELIEVE THE WORLD NEEDS TO KNOW....YOUR IMPORTANT POST ARE NOT
> > BLOCKED....SO POST WHAT YOU LIKE....
> > GLAD IM NOT YOUR STUDENT TOO
> > I GOT YOUR MAIL.....
> --
> Larry D. Farrell, Ph.D.
> Professor of Microbiology
> Idaho State University> On Nov 30, 2:01 pm, Larry Farrell
> <farrl...@isu.edu> wrote:
> >> Mitch Haynes wrote:
> >>> Why do you continue to post cytometry materials in misc.health.aids?
> >>> That is an HIV/AIDS newsgroup and your material is completely off
> >>> topic.  No one in the group is interested.
> >>> --
> >> [Multiple copies snipped]
> >> Very interesting.  I got a "Mail Undeliverable" notice when I sent
> >> this directly to Mr. Haynes but he turns around and posts multiple
> >> copies of a personal e-mail message into a newsgroup.  Extremely poor
> >> Netiquette (although it is just a further example of the poor
> >> Netiquette displayed by the issue about which I tried to contact him).
> >>     Exactly what is it that you are trying to accomplish, Mr. Haynes?
> >> --
> >> Larry D. Farrell, Ph.D.
> >> Professor of Microbiology
> >> Idaho State University

Paul Robinson was *not* informed of my message to you.  What makes
you

> think he was?

> I deleted the Undeliverable Mail notice as soon as it was received,
> assuming that it was a dead issue since you would not have received the
> message.  And then, lo and behold, you actually did.  Something is fishy!
> Why do I care?  Because you are flooding a normally useful newsgroup
> with off-topic material, wasting bandwidth and turning off people who
> might read the group.  Your behavior in this group certainly does not
> reflect well on you or on Kanecki Associates, the company for which you
> apparently work as Marketing Director.
> You have no idea how glad I am that you aren't one of my students!!
> --
> Larry D. Farrell, Ph.D.
> Professor of Microbiology
> Idaho State University

LETS GET ALL THE FACTS ABOUT THE PURDUE CYTOMETRY MAIL LIST FOR
DISCUSSIONS RELATING TO ISAC OR WHAT IS GOOD FOR J PAUL ROBINSON
PRESIDENT OF ISAC.....HEAD OF PURDUE CYTOMETRY THE ONLY LINK

http://yedda.com/people/9511561018841/

A SMALL
WITH ALL HIS POWER HE HAS BEEN ABLE TO DO WHAT EVER HE LIKES SO WHY
NOT ASK J PAUL ROBINSON PRESIDENT OF ISAC HEAD OF PURDUE CYTOMETRY.
This message: [ Message body ] [ More options ]

> Related messages: [ Next message ] [ Previous message ]
> From: VSH - Tech Support <t...@vsh.com>
> Date: Wed Jun 30 2004 - 12:08:14 EST
> David
> At the risk of sounding commercial, WinList can do what you want. I
> have
> attached a few pictures showing the WinList Region Array feature.
> With this
> you can tell the program exactly how you want the regions created and
> how
> many. If you would like to try it for yourself you can download a
> trial
> version from our web sitewww.vsh.com.
> Please let me know if you have any questions.
> Best regards
> Don
> Donald J. Herbert
> Technical Support Manager
> Verity Software House, Inc.
> PO Box 247
> 45A Augusta Road
> Topsham, ME, USA 04086
> Phone: (207) 729-6767 ext.190
> Fax: (207) 729-5443
> email: t...@vsh.com
> web:www.vsh.com

anti) Commercial Posting:)

> This message: [ Message body ] [ More options ]
> Related messages: [ Next message ] [ Previous message ]
> From: Ray Hicks <rh...@cam.ac.uk>
> Date: Wed Oct 13 2004 - 08:43:13 EST
> Just a quick nopte to let people know that FCSPress is now shareware
> (no time restrictions or feature reductions for free use, though if
> you feel like like paying for it you can using paypal). It also now
> works with Diva export files and runs ok under classic on OS 10.2 and
> 10.3. I've discontinued FCS Assistant, which has fallen behind
> FCSPress remarkably, and which failed to run as shareware on some
> systems (it insisted you buy it or refused to run - FCSPress doesn't
> even have a nag screen now - pesky things).
> FCSPress is available fromwww.fcspress.com
> Enjoy!
> Ray

From: VSH Tech Support [mailto:T...@vsh.com]

> > Sent: Wednesday, February 21, 2007 10:30 AM
> > To: Cytometry Mailing List
> > Subject: RE: DNA analysis software
> > Hello Ibtissam,
> > ModFit LT, for PC or Mac, has advanced modeling capability for
> > research applications in DNA cell cycle analysis. You may use any of
> > the model templates the program offers, or create your own models for
> > non-traditional analysis, including non-mammalian DNA cell cycle
> > studies. ModFit LT can be linked to our WinList program to provide a
> > complete cell cycle analysis on any number of sub-populations with a
> > single click of a button.
> > For an overview, visithttp://www.vsh.com/products%a0.
> > Best regards,
> > Don
> > Donald J. Herbert
> > Technical Support Manager
> > Verity Software House

FW: FlowJo Seminar for Los Angeles Basin
From: Barsky, Lora (LBar...@chla.usc.edu)
Date: Tue Mar 20 2001 - 14:28:04 EST

Next message: PLo...@adarc.org: "Apoptosis, Annexin V and Ficoll"
Previous message: Olson, Douglas: "CCR7 Available"
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Reply: Sue DeMaggio: "Re: FW: FlowJo Seminar for Los Angeles Basin"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]

I'm not sure if there is a Los Angeles Basin users
group,
but thought I could advertise here to interested parties.
Thanks,

Lora Barsky - Lead Operator
Research Immunology/BMT FACS Core
Childrens Hospital Los Angeles

On Aug 20, 2004, at 2:25 PM, J. Paul Robinson wrote:

> > > ummm....Mario says..
> > > "In life sciences -- particularly in research life sciences --
> > > probably 50 to 70% of research laboratories used
> > > Macs"....while I have a passionate dislike for Windows......is
> > > this really true ??? or is the key word there "used"?? (Ok...I
> > > have put on my helmet and armor....waiting...)
> > > paul
> > >> For all of the mr groupies out there in cytometry cyberspace. Don't
> > >> wet your
> > >> pocket protectors over this.
> > >> Honestly though, well deserved praise for Mario & the Tree Star group:
> > >>http://www.apple.com/science/profiles/ro...

Purdue Cytometry Mailing List: Re: Thanks for the suggestions -
... http://www.wehi.edu.au/cytometry/WEASELv... > > Winlist 3d
from
Verity House Software (www.vsh.com) ... Yes, I live off FlowJo sales,
and that's a blatantly ...
http://www.cyto.purdue.edu/hmarchiv/2006... - 8.5KB
71%

From: Adam Treister (a...@treestar.com)
Date: Mon Apr 22 2002 - 00:15:07 EST
* Reply: Mario Roederer: "Job Opening -- Immediate -- Vaccine

______________________________________...
Only two more weeks until ISAC, that biennial bacchanalia of flower
power
and fun! So I hope you'll excuse a bunch of blatantly commercial
announcements to the list, but endulge me this one time and read on.

At the show we'll be releasing FlowJo Version 4. We've got new
platforms
for overlaying and clustering, we've made it work better across the
Internet, added all sorts of new

For those who haven't found it yet, we've unveiled a spanking new
FlowJo
website. Flowjo.com is chuck full of new content, functionality and
spunk.
Automated price quotes, online ordering, a FAQ that will guide you to
new
depths of understanding, and none of that awful yellow on black text.
The
search engine even works. No ads & cookie-free. Check it out.
Specifically, you should check our pricing. Prices are going up on
May 10.
It has been a number of years since we've changed our prices and with
the
development of the OSX and PC versions, it¹s time for a leap. I
guess
there's no such thing as a free launch. Anyway, this may be a great
time to
buy that FlowJo ten pack you've been thinking about. Licenses
purchased
before May 10 are entitled to a year of free upgrades, including the
4.0
release.
Unleash the flower power!
Adam
--
Adam Treister
Tree Star,